SHARMA GLOBAL BIOTECH LABORATORY

REGISTRATION FEE: 500/-

MODULE 1 : 1MONTH                           

BIOCHEMISTRY                                                       

1. Separation of amino acids and plant pigments by paper chromatography and thin layer chromatography.
2. Estimation of chlorophyll content.
3. Estimation of carbohydrate content
4. Estimation of protein content
5. UV absorption of amino acids and proteins
6. To determine an unknown protein concentration by plotting a standard graph of BSA using UV-VIS spectrophotometer and validating the Beer-Lambert’s law.
7. Estimation of DNA by DPA method.
8. Estimation of RNA by Orcinol method.

MODULE 2: 1MONTH                           

MOLECULAR BIOLOGY

DNA ISOLATION AND AMPLIFICATION            

1. Preparation of reagents
2. Isolation of genomic DNA from plant and yeast cells.
3. Isolation of genomic DNA from bacteria.
4. Isolation of plasmid from bacteria.
5. PCR amplification of DNA.
6. Agarose gel electrophoresis of DNA.

MODULE 3: 1MONTH 

PROTEIN ISOLATION AND SDS PAGE                                      

1. Reagent preparation.
2. Separation of crude protein samples
3. Ion exchange chromatography
4. SDS-PAGE

MODULE 4 : 1MONTH                           

CELL BIOLOGY                                              

1. Preparation of fixative, preservation and acetocarmine stain for cytological squashes and smears.
2. To study various stages of mitosis and meiosis.
3. Isolation of chloroplast.

MODULE 5: 1MONTH                                         

MICROBIOLOGY

1. Preparation of different types of media.
2. Isolation and maintenance of pure culture of microorganism

1. Serial dilution
2. Pour plate method
3. Spread plate method
4. Streak plate method

3. Microscopic examination of bacteria

1. Gram staining
2. Spore staining
3. Fungi staining (spore and body)
4. Eukaryotic microbial staining

4. Growth curve of bacteria
5. Effect of environmental factors on growth of bacteria

• Salt
• Ph
• Temperature

6. Viable count of bacteria from soil sample
7. Biochemical characterization of microbes
8. Immobilization of bacterial cells
9. Effect of antibiotics on various Gram positive and Gram negative bacteria
10. Minimum Inhibitory Concentration (MIC) and Anti Sensitivity Test (AST)
11. Isolation of rhizobium species from root nodules.

MODULE 6: 1MONTH                           

PLANT BIOTECHNOLOGY                                     

1. Preparation of media.
2.  Surface sterilization of explant.
3. Selection, preparation and inoculation of explant for callusing.
4. Sub-culturing of callus in differential media.
5. Sub-culturing callus for suspension culture.
6. Regeneration of plantlets
7. Field transfer after hardening
8. Micropropagation- large number of production of plantlets through apical tissue culture.

TRAINING PROGRAM

1 Month, 2 months, 3 months and 6 months

REGISTRATION FEE: 500/-

NOTE: CERTIFICATES WILL BE PROVIDED.